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monosodium urate msu crystals  (InvivoGen)


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    InvivoGen monosodium urate msu crystals
    Monosodium Urate Msu Crystals, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monosodium+urate+crystals+msu/MSU+Crystals/pm42202946-50-38-44
    Average 96 stars, based on 327 article reviews
    monosodium urate msu crystals - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Inhibition:

    Article Title: Dihydrooxazole and thiourea derivatives modulating the NLRP3 inflammasome pathway
    Article Snippet: .. 424.2 Biological Assay Description NLRP3 Inhibition Assay The following assays were used to determine the inhibitory activity of test compounds on the NLRP3 inflammasome pathway using common stimuli Nigericin (Invivogen) or monosodium urate crystals (MSU) (Invivogen). .. Cell Culture Human monocyte-like cells were cultured in RPMI-1640 Glutamax medium supplemented with 10% heat inactivated FCS and 50 U/ml penicillin-streptomycin (Life Technologies).

    Activity Assay:

    Article Title: Dihydrooxazole and thiourea derivatives modulating the NLRP3 inflammasome pathway
    Article Snippet: .. 424.2 Biological Assay Description NLRP3 Inhibition Assay The following assays were used to determine the inhibitory activity of test compounds on the NLRP3 inflammasome pathway using common stimuli Nigericin (Invivogen) or monosodium urate crystals (MSU) (Invivogen). .. Cell Culture Human monocyte-like cells were cultured in RPMI-1640 Glutamax medium supplemented with 10% heat inactivated FCS and 50 U/ml penicillin-streptomycin (Life Technologies).

    Activation Assay:

    Article Title: Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation
    Article Snippet: .. To induce NLRP3 inflammasome activation, macrophages were first primed with 300 ng/mL LPS (E. coli 0111; B4, Sigma-Aldrich) before stimulated with 5 mM ATP (Sigma-Aldrich) for 15 min to 120 min, 10 μM nigericin (InvivoGen) for 1 h, 0.6 mg/mL monosodium urate crystals (MSU) (InvivoGen) for 6 h in RPMI-1640 medium, as indicated in the figure legends. .. To assess the AIM2 inflammasome, BMDMs were first primed with 300 ng/mL LPS for 3 h before stimulated with poly (dA:dT) (2.5 μg/mL, InvivioGen) complexed with transfection reagent LyoVec for 7 h. To inhibit PDHK, macrophages were pretreated for 30 min before LPS priming or after LPS priming (together with inflammasome stimuli as described above) with DCA (5–30 mM, Sigma-Aldrich), JX06 (5–40 mM, Rocris) for an indicated time as described in the figure legends.

    Article Title: Pyruvate Dehydrogenase Kinase Supports Macrophage NLRP3 Inflammasome Activation During Acute Inflammation
    Article Snippet: .. To induce NLRP3 inflammasome activation, macrophages were first primed with 300 ng/ml LPS (E. coli 0111; B4, Sigma-Aldrich) before stimulated with 5 mM ATP (Sigma-Aldrich) for 15 min to 120 min, 10 M nigericin (InvivoGen) for 1 h, 0.6 mg/ml monosodium urate crystals (MSU) (InvivoGen) for 6 h, as indicated in the figure legends. .. To inhibit PDHK, macrophages were pretreated for 30 min before LPS priming or after LPS priming (together with the NLPR3 inflammasome stimuli as described above) with DCA (5-30 mM, Sigma-Aldrich), JX06 (5-40 M, Rocris) for an indicated time as described in the figure legend.

    Article Title: Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation
    Article Snippet: .. To induce NLRP3 inflammasome activation, macrophages were first primed with 300 ng/ml LPS (E. coli 0111; B4, Sigma-Aldrich) before stimulated with 5 mM ATP (Sigma-Aldrich) for 15 min to 120 min, 10 μM nigericin (InvivoGen) for 1 h, 0.6 mg/ml monosodium urate crystals (MSU) (InvivoGen) for 6 h, as indicated in the figure legends. .. To inhibit PDHK, macrophages were pretreated for 30 min before LPS priming or after LPS priming (together with the NLPR3 inflammasome stimuli as described above) with DCA (5-30 mM, Sigma-Aldrich), JX06 (5-40 μM, Rocris) for an indicated time as described in the figure legend.

    Article Title: Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation.
    Article Snippet: .. To induce NLRP3 inflammasome activation, macrophages were first primed with 300 ng/mL LPS (E. coli 0111; B4, Sigma-Aldrich) before stimulated with 5 mM ATP (Sigma-Aldrich) for 15 min to 120 min, 10 mM nigericin (InvivoGen) for 1 h, 0.6 mg/mL monosodium urate crystals (MSU) (InvivoGen) for 6 h in RPMI-1640medium, as indicated in the figure legends. .. To assess the AIM2 inflammasome, BMDMs were first primed with 300 ng/mL LPS for 3 h before stimulated with poly (dA:dT) (2.5 mg/mL, InvivioGen) complexed with transfection reagent LyoVec for 7 h. To inhibit PDHK, macrophages were pretreated for 30 min before LPS priming or after LPS priming (together with inflammasome stimuli as described above) with DCA (5–30mM, Sigma-Aldrich), JX06 (5–40 mM, Rocris) for an indicated time as described in the figure legends.

    Concentration Assay:

    Article Title: CCDC50 suppresses NLRP3 inflammasome activity by mediating autophagic degradation of NLRP3.
    Article Snippet: The NLRP3-directed inflammasome complex is crucial for the host to resist microbial infection and monitor cellular damage.. However, the hyperactivation of NLRP3 inflammasome is implicated in pathogenesis of inflammatory diseases, including inflammatory bowel disease (IBD).. Autophagy and autophagy-related genes are closely linked to NLRP3-mediated inflammation in these inflammatory disorders.

    other:

    Article Title: Non-decameric NLRP3 forms an MTOC-independent inflammasome
    Article Snippet: Phorbol 12-myristate 13-acetate (PMA), LPS ultrapure from Escherichia coli K12 (LPS-EK, tlr-peklps), nigericin sodium salt (tlr-nig), imiquimod (tlrl-imq) and monosodium urate crystals (MSU) were obtained from InvivoGen.



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    InvivoGen monosodium urate msu crystals
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    monosodium urate msu tlrl msu  (InvivoGen)
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    Effect of depleting discrete protein subsets on the ability of SD-supts to induce NETs. Human neutrophils adherent to poly-L-lysine-coated coverslips were stimulated with 1 mg/mL <t>monosodium</t> urate <t>(MSU)</t> for 2.5 h. The culture supernatant was collected and depleted of the original stimulus, yielding a stimulus-depleted culture supernatant (SD-supt). These SD-supts were then pre-cleared and immunoprecipitated using pan-histone (“histones”) or pan-S100 proteins (“S100”) antibodies as described in Methods. Alternatively, SD-supts were pre-cleared, mixed with rh sRAGE, and immunoprecipitated using anti-sRAGE antibodies (“sRAGE”). The resulting supernatants from immunodepleted SD-supts were stored and later used as a NET stimulus. Human neutrophils adherent to poly-L-lysine-coated coverslips were incubated for 4 h at 37 °C in the absence of stimuli (“unstim”) or in the presence of SD-supts that had been only pre-cleared (“isotype”) or immunodepleted of S100 proteins, histones, or sRAGE-bound proteins. NET formation was then assessed by microscopy and standardized NET indices were calculated. A representative experiment is shown (right panel), along with compiled data (mean ± s.e.m.) from at least 3 independent experiments. **, p < 0.01; ***, p< 0.001 vs the positive control; using Student’s paired t test.
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    monosodium urate crystals  (InvivoGen)
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    Effect of depleting discrete protein subsets on the ability of SD-supts to induce NETs. Human neutrophils adherent to poly-L-lysine-coated coverslips were stimulated with 1 mg/mL <t>monosodium</t> urate <t>(MSU)</t> for 2.5 h. The culture supernatant was collected and depleted of the original stimulus, yielding a stimulus-depleted culture supernatant (SD-supt). These SD-supts were then pre-cleared and immunoprecipitated using pan-histone (“histones”) or pan-S100 proteins (“S100”) antibodies as described in Methods. Alternatively, SD-supts were pre-cleared, mixed with rh sRAGE, and immunoprecipitated using anti-sRAGE antibodies (“sRAGE”). The resulting supernatants from immunodepleted SD-supts were stored and later used as a NET stimulus. Human neutrophils adherent to poly-L-lysine-coated coverslips were incubated for 4 h at 37 °C in the absence of stimuli (“unstim”) or in the presence of SD-supts that had been only pre-cleared (“isotype”) or immunodepleted of S100 proteins, histones, or sRAGE-bound proteins. NET formation was then assessed by microscopy and standardized NET indices were calculated. A representative experiment is shown (right panel), along with compiled data (mean ± s.e.m.) from at least 3 independent experiments. **, p < 0.01; ***, p< 0.001 vs the positive control; using Student’s paired t test.
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    Effect of depleting discrete protein subsets on the ability of SD-supts to induce NETs. Human neutrophils adherent to poly-L-lysine-coated coverslips were stimulated with 1 mg/mL <t>monosodium</t> urate <t>(MSU)</t> for 2.5 h. The culture supernatant was collected and depleted of the original stimulus, yielding a stimulus-depleted culture supernatant (SD-supt). These SD-supts were then pre-cleared and immunoprecipitated using pan-histone (“histones”) or pan-S100 proteins (“S100”) antibodies as described in Methods. Alternatively, SD-supts were pre-cleared, mixed with rh sRAGE, and immunoprecipitated using anti-sRAGE antibodies (“sRAGE”). The resulting supernatants from immunodepleted SD-supts were stored and later used as a NET stimulus. Human neutrophils adherent to poly-L-lysine-coated coverslips were incubated for 4 h at 37 °C in the absence of stimuli (“unstim”) or in the presence of SD-supts that had been only pre-cleared (“isotype”) or immunodepleted of S100 proteins, histones, or sRAGE-bound proteins. NET formation was then assessed by microscopy and standardized NET indices were calculated. A representative experiment is shown (right panel), along with compiled data (mean ± s.e.m.) from at least 3 independent experiments. **, p < 0.01; ***, p< 0.001 vs the positive control; using Student’s paired t test.
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    Image Search Results


    Effect of depleting discrete protein subsets on the ability of SD-supts to induce NETs. Human neutrophils adherent to poly-L-lysine-coated coverslips were stimulated with 1 mg/mL monosodium urate (MSU) for 2.5 h. The culture supernatant was collected and depleted of the original stimulus, yielding a stimulus-depleted culture supernatant (SD-supt). These SD-supts were then pre-cleared and immunoprecipitated using pan-histone (“histones”) or pan-S100 proteins (“S100”) antibodies as described in Methods. Alternatively, SD-supts were pre-cleared, mixed with rh sRAGE, and immunoprecipitated using anti-sRAGE antibodies (“sRAGE”). The resulting supernatants from immunodepleted SD-supts were stored and later used as a NET stimulus. Human neutrophils adherent to poly-L-lysine-coated coverslips were incubated for 4 h at 37 °C in the absence of stimuli (“unstim”) or in the presence of SD-supts that had been only pre-cleared (“isotype”) or immunodepleted of S100 proteins, histones, or sRAGE-bound proteins. NET formation was then assessed by microscopy and standardized NET indices were calculated. A representative experiment is shown (right panel), along with compiled data (mean ± s.e.m.) from at least 3 independent experiments. **, p < 0.01; ***, p< 0.001 vs the positive control; using Student’s paired t test.

    Journal: Frontiers in Immunology

    Article Title: A feedback loop sustaining neutrophil extracellular trap formation involves S100 proteins, histones, TLR2 and RAGE, and is restrained by albumin

    doi: 10.3389/fimmu.2026.1774475

    Figure Lengend Snippet: Effect of depleting discrete protein subsets on the ability of SD-supts to induce NETs. Human neutrophils adherent to poly-L-lysine-coated coverslips were stimulated with 1 mg/mL monosodium urate (MSU) for 2.5 h. The culture supernatant was collected and depleted of the original stimulus, yielding a stimulus-depleted culture supernatant (SD-supt). These SD-supts were then pre-cleared and immunoprecipitated using pan-histone (“histones”) or pan-S100 proteins (“S100”) antibodies as described in Methods. Alternatively, SD-supts were pre-cleared, mixed with rh sRAGE, and immunoprecipitated using anti-sRAGE antibodies (“sRAGE”). The resulting supernatants from immunodepleted SD-supts were stored and later used as a NET stimulus. Human neutrophils adherent to poly-L-lysine-coated coverslips were incubated for 4 h at 37 °C in the absence of stimuli (“unstim”) or in the presence of SD-supts that had been only pre-cleared (“isotype”) or immunodepleted of S100 proteins, histones, or sRAGE-bound proteins. NET formation was then assessed by microscopy and standardized NET indices were calculated. A representative experiment is shown (right panel), along with compiled data (mean ± s.e.m.) from at least 3 independent experiments. **, p < 0.01; ***, p< 0.001 vs the positive control; using Student’s paired t test.

    Article Snippet: Among neutrophil stimuli, monosodium urate (MSU) (tlrl-msu) and ultra-pure peptidoglycan (PGN) were from Invivogen (#tlrl-pgnb3); TNFα (#210-TA) and GM-CSF (#7954-GM) were from R&D Systems; and N-formyl-methionyl-phenylalanine (fMLP) was from Millipore Sigma (#F3506).

    Techniques: Immunoprecipitation, Incubation, Microscopy, Positive Control